A new approach to transfect NF-kappa B decoy oligodeoxynucleotides into the periodontal tissue using the ultrasound-microbubble method

作者：Yamaguchi Hiroyuki; Ishida Yuji^*; Hosomichi Jun; Suzuki Jun ichi; Usumi Fujita Risa; Shimizu Yasuhiro; Kaneko Sawa; Ono Takashi

来源：International Journal of Oral Science, 2017, 9(2): 80-86.

DOI：10.1038/ijos.2017.10

• decoy oligodeoxynucleotide
• gene therapy
• periodontal tissue
• ultrasound

The objective of this study is to investigate the effect of the ultrasound-microbubble technique in nuclear factor kappa B (NF-kappa B) decoy oligodeoxynucleotide (ODN) transfection in the gingival tissue in mice. The 6-FAM-labeled scrambled decoy ODN with microbubbles was applied to the periodontal tissue in 8-week-old male C57BL/6J mice by ultrasound radiation at low (LUM-Sc) and high (HUM-Sc) intensities to optimize the transfection condition of the ultrasound-microbubble method. Histological inspections were performed two hours after transfection to compare the expression with that in the sham-operated group without ultrasound radiation (A-Sc). Then, an NF-kappa B decoy was transfected into the periodontal tissue using the highintensity ultrasound-microbubble (HUM-NF) technique to examine the anti-inflammatory effects of the decoy ODN. Western blot analysis was performed to investigate the expression of interleukin(IL)-1 beta, IL-6 and intercellular adhesion molecule-1 (ICAM-1) in the gingival tissues in the HUM-Sc, the HUM-NF and control groups. The fluorescence microscopy results showed that the fluorescent intensity in the periodontal tissues in the LUM-Sc and HUM-Sc groups was significantly higher than that in the A-Sc and the control groups. The fluorescent intensity in the HUM-Sc group, especially in the gingival connective tissue, was the highest of all groups. Western blot analysis indicated that the protein expression levels of IL-1 beta, IL-6 and ICAM-1 in the HUM-NF group were significantly lower than those in the HUM-Sc and the control groups. These findings suggest that the high-intensity ultrasound-microbubble technique is an effective tool for decoy transfection into the periodontal tissue.

• 出版日期2017-6