A unique human blood-derived cell population displays high potential for producing insulin

作者：Zhao Yong^*; Huang Zhihua; Lazzarini Ping; Wang Yong; Di Anke; Chen Meiling

来源：Biochemical and Biophysical Research Communications, 2007, 360(1): 205-211.

DOI：10.1016/j.bbrc.2007.06.035

• diabetes
• insulin-producing cell
• blood
• stem cell

Blood can provide a valuable source for the generation of stem cells. Herein we identified a novel cell population from adult human blood, designated peripheral blood insulin-producing cells (PB-IPC). Phenotypic analysis demonstrated that PB-IPC displayed the embryonic stem (ES) cell-associated transcription factors including Oct-4 and Nanog, along with the hematopoietic markers CD9, CD45, and CD117; but lacked expression of the hematopoietic stem cell marker CD34 as well as lymphocyte and monocyte/macrophage markers. Notably, in vitro and it? vivo characterization revealed that PB-IPC demonstrated characteristics of islet P cell progenitors including the expression of cell-specific insulin gene transcription factors and prohormone convertases, production of insulin, formation of insulin granules, and the ability to reduce hyperglycemia and migrate into pancreatic islets after transplantation into the diabetic mice. These findings may open up new avenues for autologous blood stem cell-based therapies for diabetes.

• 出版日期2007-8-17