Epizootology and experimental infection of Yokose virus in bats

作者：Watanabe Shumpei; Omatsu Tsutomu; Miranda Mary E G; Masangkay Joseph S; Ueda Naoya; Endo Maiko; Kato Kentaro; Tohya Yukinobu; Yoshikawa Yasuhiro; Akashi Hiroomi^*

来源：Comparative Immunology, Microbiology and Infectious Diseases, 2010, 33(1): 25-36.

DOI：10.1016/j.cimid.2008.07.008

• ELISA
• Yokose virus
• Bat
• Experimental infection
• Anti-bat IgG
• Chiroptera

To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.

• 出版日期2010-1