Novel process for producing 6-deoxy monosaccharides from L-fucose by coupling and sequential enzymatic method

作者：Shompoosang Sirinan; Yoshihara Akihide; Uechi Keiko; Asada Yasuhiko; Morimoto Kenji^*

来源：Journal of Bioscience and Bioengineering, 2016, 121(1): 1-6.

DOI：10.1016/j.jbiosc.2015.04.017

• L-Fucose
• 6-Deoxy-L-talose
• 6-Deoxy-L-sorbose
• 6-Deoxy-L-gulose
• 6-Deoxy-L-idose
• D-Arabinose isomerase
• L-Rhamnose isomerase
• D-Allulose 3-epimerase
• L-Ribose isomerase
• D-Glucose isomerase

We biosynthesized 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose, which rarely exist in nature, from L-fucose by coupling and sequential enzymatic reactions. The first product, 6-deoxy-L-talose, was directly produced from L-fucose by the coupling reactions of immobilized D-arabinose isomerase and immobilized L-rhamnose isomerase. In one-pot reactions, the equilibrium ratio of L-fucose, L-fuculose, and 6-deoxy-L-talose was 80:9:11. In contrast, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose were produced from L-fucose by sequential enzymatic reactions. D-Arabinose isomerase converted L-fucose into L-fuculose with a ratio of 88:12. Purified L-fuculose was further epimerized into 6-deoxy-L-sorbose by D-allulose 3-epimerase with a ratio of 40:60. Finally, purified 6-deoxy-L-sorbose was isomerized into both 6-deoxy-L-gulose with an equilibrium ratio of 40:60 by L-ribose isomerase, and 6-deoxy-L-idose with an equilibrium ratio of 73:27 by D-glucose isomerase. Based on the amount of L-fucose used, the production yields of 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose were 7.1%, 14%, 2%, and 2.4%, respectively.

• 出版日期2016-1