Determination of prolactin hormone in serum and urine using an electrochemical immuno sensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes

作者：Serafin V; Aguei L; Yanez Sedeno P^*; Pingarron J M

来源：Sensors and Actuators B: Chemical , 2014, 195: 494-499.

DOI：10.1016/j.snb.2014.01.055

• Electrochemical immunosensor
• Prolactin
• Urine
• Human serum
• Poly(pyrrolepropionic acid)
• Hybrid nanomaterials

A novel electrochemical immunosensor for the determination of the hormone prolactin (PRL) is reported by using glassy carbon electrodes modified with a poly(pyrrolepropionic acid)/multiwalled carbon nanotubes hybrid nanomaterial as transducer. An indirect competitive assay was utilized where the antigen was covalently immobilized on the modified electrode and a fixed amount of anti-PRL labelled with alkaline phosphatase (AP) was reacted with PRL in the sample. After attachment of the remaining labelled antibody to the immobilized PRL, the affinity reaction was monitored by differential pulse voltammetry of the AP enzyme reaction product using 1-naphtyl phosphate as substrate. Upon optimization of the variables affecting the immunosensor behaviour, a calibration graph for PRL with a wide linear range between 10(-2) and 10(4) ng/mL was obtained. The calculated LOD value, 3 pg/mL resulted to be remarkably lower than those reported previously using other approaches. Moreover, excellent reproducibility of the voltammetric measurements and selectivity against other hormones was found. The immunosensor was successfully applied to the analysis of human serum and urine at the clinically relevant concentration levels.

• 出版日期2014-5