Analysis of p53-RNA interactions in cultured human cells

作者：Riley Kasandra J L; Maher L James III^*

来源：Biochemical and Biophysical Research Communications, 2007, 363(2): 381-387.

DOI：10.1016/j.bbrc.2007.08.181

• p53
• RNA
• UV-cross-linking
• formaldehyde
• CLIP

Tumor suppressor p53 is a well-characterized transcription factor that binds DNA. More enigmatic are the RNA-binding properties of p53 and their physiological relevance. We used three sensitive co-immunoprecipitation methods in an attempt to detect RNAs that tightly associate with p53 in cultured human cells. Although recombinant p53 protein binds RNA in a sequence-nonspecific mode, we do not detect specific in vivo RNA binding by p53. These results suggest that RNA binding is prevented by post- translational p53 modifications. A ribonucleoprotein (not p53) is purified by multiple IgG monoclonal antibodies (including anti-p53 antibodies) from both p53 +/+ and p53 null cells. Caution is therefore required in interpreting RNA co-immunoprecipitation experiments. Though not formally excluded, these results do not support models in which p53 binds specific RNA partners in vivo.

• 出版日期2007-11-16