Downregulation of Kir6.1/SUR2B channels in the obese rat aorta

作者：Fan Li Hong; Tian Hong Yan; Wang Jun; Huo Jian Hua; Hu Zhi; Ma Ai Qun^*; Cao Yong Xiao

来源：Nutrition, 2009, 25(3): 359-363.

DOI：10.1016/j.nut.2008.09.005

• Kir6.1
• SUR2B
• Hypertension
• Obesity
• Aorta

Objective: This study was designed to evaluate the contribution of adenosine triphosphate-dependent potassium channels to the increase in blood pressure observed in obese rats.
Methods: The experiment was performed in male Sprague-Dawley rats. Glibenclamide-sensitive currents were measured in vascular smooth muscle cells by patch-clamp. Expressions of Kir6.1 and SUR2B were examined by reverse transcription polymerase chain reaction and western blot techniques, respectively.
Results: In the aortic vascular smooth muscle cells, pinacidil induced glibenclamide-sensitive currents. The current from obese rats was significantly lower (-10.55 /- 1.63 pA/pF) compared with that from the control rats (-20.18 /- 2.79 pA/pF). Expressions of Kir6.1 and SUR2B were downregulated in vascular smooth muscle cells of aortas from the obese rats.
Conclusion: These findings suggest that the adenosine triphosphate-dependent potassium channel is downregulated in smooth muscle cells from the aortas of obese rats, which may contribute to the increase in blood pressure in these rats.

• 出版日期2009-3
• 单位西安交通大学