A comprehensive analysis of constitutive naturally processed and presented HLA-C*04:01 (Cw4)-specific peptides

作者：Schittenhelm R B; Dudek N L; Croft N P; Ramarathinam S H; Purcell A W^*

来源：Tissue Antigens, 2014, 83(3): 174-179.

DOI：10.1111/tan.12282

• HLA-Cw4
• HLA-C*04:01
• HLA-B*35:03
• mass spectrometry
• immunopeptidome
• antigen presentation
• C1R cells

The human B lymphoblastoid cell line C1R is widely regarded as human leukocyte antigen-A (HLA-A)/HLA-B negative and is therefore frequently exploited as a recipient cell line to study HLA class I functions. However, the normal levels of HLA-C*04:01 often hamper the investigation of introduced HLA class I allomorphs, which is particularly evident in sensitive applications such as mass spectrometry. Here we describe the comprehensive analysis of endogenous HLA-C*04:01 ligands expressed on the surface of C1R cells to (i) define a large sequence dataset of HLA-C*04:01 ligands, to (ii) refine the HLA-C*04:01 peptide-binding motif and (iii) to provide a resource that allows discrimination between peptides bound to introduced HLA class I subtypes and to the endogenous HLA-C*04:01 molecules.

• 出版日期2014-3