An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons

作者：Meng, X. Y.; Li, Y. S.; Zhou, Y.^*; Zhang, Y. Y.; Yang, L.; Qiao, B.; Wang, N. N.; Hu, P.; Lu, S. Y.; Ren, H. L.; Liu, Z. S.; Zhang, J. H.; Wang, X. R.

来源：Analytical Biochemistry, 2015, 473: 1-6.

DOI：10.1016/j.ab.2014.12.002

• Pyrene
• Monoclonal antibody
• ELISA
• Water

Polycyclic aromatic hydrocarbons (PAHs) can form DNA-binding compounds that show genotoxicity and carcinogenicity. Pyrene, as a PAH, was covalently linked to carrier protein bovine serum albumin and ovalbumin. A monoclonal antibody (McAb) was produced that showed high cross-reactivity values with chrysene (169.73%), benzo[a]pyrene (693.34%), benzo[a]anthracene (16.36%), and indeno[1,2, 3-cd]pyrene (40.96%) and showed no significant cross-reactivity values with other homologues (<0.1%). A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of pyrene and some homologues in water samples. The detection limit of the assay was 65.08 pg ml(-1). The average recoveries of PAHs from tap water, lake water, and mineral water were 99.13, 99.74, and 99.19%, respectively, indicating that matrices of water samples do not interfere with the assay. The results demonstrated that the developed ELISA seems to be a potential method for monitoring of pyrene and some homologous PAHs in water samples.

• 出版日期2015-3-15
• 单位吉林大学