nVesicle miR-195 derived from Endothelial Cells Inhibits Expression of Serotonin Transporter in Vessel Smooth Muscle Cells

作者：Gu, Junzhong; Zhang, Huiyuan; Ji, Bingyang; Jiang, Hui; Zhao, Tao; Jiang, Rongcai; Zhang, Zhiren; Tan, Shengjiang; Ahmed, Asif; Gu, Yuchun^*

来源：Scientific Reports, 2017, 7(1): 43546.

DOI：10.1038/srep43546

• DENDRITIC CELLS
• PULMONARY-HYPERTENSION
• MICRORNAS
• EXOSOMES
• RECEPTOR
• PATHWAY
• GENE

Serotonin or 5-hydroxytryptamine (5-HT) has been shown to be essential in lots of physiological and pathological processes. It is well known that 5-HT and 5-HT transporter (5-HTT) play important roles in the pulmonary artery in pulmonary hypertension. However, little is known about the function of 5-HTT in other arteries. In this study we found that the expression of 5-HTT was elevated in injured carotid arteries and over-expression of 5-HTT induced proliferation of smooth muscle cells (SMCs); however, this phenotype could be reversed by knocking-down of 5-HTT or endothelial cells conditional medium (EC-CM). A 5-HTT inhibitor, fluoxetine, treated animals also exhibited reduced restenosis after injury. We identified that miR-195 was packaged in the extracellular vesicles from EC-CM. We further confirmed that extracellular vesicles could transfer miR-195 from ECs to SMCs to inhibit the expression of 5-HTT in SMCs and the proliferation of SMCs. These results provide the first evidence that ECs communicate with SMCs via micro-RNA195 in the regulation of the proliferation of SMCs through 5-HTT, which will contribute to a better understanding of communications between ECs and SMCs via micro-RNA. Our findings suggest a potential target for the control of vessel restenosis.

• 出版日期2017-3-8
• 单位北京协和医学院; 河北医科大学; 中国医学科学院北京协和医院; 北京大学; 哈尔滨医科大学; 苏州大学; 中国医学科学院阜外医院; 宿州学院; 天津市环湖医院