Hyaluronic Acid Conjugates of TLR7/8 Agonists for Targeted Delivery to Secondary Lymphoid Tissue

作者：Yoo Euna; Salyer Alex C D; Brush Michael J H; Li Yupeng; Trautman Kathryn L; Shukla Nikunj M; De Beuckelaer Ans; Lienenklaus Stefan; Deswarte Kim; Lambrecht Bart N; De Geest Bruno G; David Sunil A^*

来源：Bioconjugate Chemistry, 2018, 29(8): 2741-2754.

DOI：10.1021/acs.bioconjchem.8b00386

Immunogens carried in lymphatic fluid drain via afferent vessels into regional lymph nodes and facilitate the efficient induction of appropriate immune responses. The lymphatic system possesses receptors recognizing hyaluronic acid (HA). Covalent conjugates of small-molecule TLR7/8 agonists with HA are entirely devoid of immunostimulatory activity in vitro. In murine models of immunization, however, such conjugates traffic to lymph nodes, where they are "unmasked", releasing the small molecule TLR7/8 agonist from the carrier polysaccharide. The resulting focal immunostimulation is manifested in potent adjuvantic effects with negligible systemic exposure. The efficient delivery of immunogens has been a major challenge in the development of subunit vaccines, and enhancing targeted delivery of immunogens to secondary lymphoid organs might be a promising approach for improving vaccine efficacy, as well as safety.

• 出版日期2018-8