An investigation on the effect of beta-CD modified Fe3O4 magnetic nanoparticles on aggregation of amyloid b peptide (25-35)

作者：Ansari Mojtaba; Habibi Rezaei Mehran^*; Salahshour Kordestani Soheila^*; Ferdousi Maryam; Movahedi Ali Akbar Moosavi

来源：Materials Technology, 2016, 31(6): 315-321.

DOI：10.1179/17535557B15Y.000000002

• Alzheimer's disease
• Fibrilization
• Aggregation
• beta-CD-Fe3O4 MNPs
• Core-shell structures

In this study, beta-cyclodextrin (beta-CD)-functionalised Fe3O4 composite nanoparticles with core-shell structures were fabricated via carbodiimide activation, which were employed to interact with amyloid b (25-35) peptides (A beta(25-35)). The functionalised magnetic core-shell nanoparticles (MNPs) were characterised by Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetric analysis (TGA) and X-ray Diffraction, which verified that beta-CD was successfully grafted on the surface of Fe3O4 MNPs. The effect of beta-CD grafted on the Fe3O4 MNPs on the aggregation of amyloid-beta-(25-35) peptides were investigated by atomic force microscopy (AFM) and Thioflavin T fluorescence measurements. The result showed that, without functionalized MNPs, the amyloid (25-35) peptides aggregated gradually from monomers and oligomers to long fibrils with the incubation time. In comparison, modified MNPs dramatically inhibited fibrilization and further aggregation. This study may contribute to the development of new diagnostic and therapeutic strategies against amyloid-related diseases.

• 出版日期2016