A new paradigm for transcription factor TFIIB functionality

作者：Gelev Vladimir; Zabolotny Janice M; Lange Martin; Hiromura Makoto; Yoo Sang Wook; Orlando Joseph S; Kushnir Anna; Horikoshi Nobuo; Paquet Eric; Bachvarov Dimcho; Schaffer Priscilla A; Usheva Anny^*

来源：Scientific Reports, 2014, 4(1): 3664.

DOI：10.1038/srep03664

Experimental and bioinformatic studies of transcription initiation by RNA polymerase II (RNAP2) have revealed a mechanism of RNAP2 transcription initiation less uniform across gene promoters than initially thought. However, the general transcription factor TFIIB is presumed to be universally required for RNAP2 transcription initiation. Based on bioinformatic analysis of data and effects of TFIIB knockdown in primary and transformed cell lines on cellular functionality and global gene expression, we report that TFIIB is dispensable for transcription of many human promoters, but is essential for herpes simplex virus-1 (HSV-1) gene transcription and replication. We report a novel cell cycle TFIIB regulation and localization of the acetylated TFIIB variant on the transcriptionally silent mitotic chromatids. Taken together, these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects.

• 出版日期2014-1-20