A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin

作者：Shen, Haicong; Xu, Fei; Xiao, Meng; Fu, Qiangqiang; Cheng, Zhenzhu; Zhang, Shiwei; Huang, Caihong; Tang, Yong^*

来源：Analyst, 2017, 142(23): 4393-4398.

DOI：10.1039/c7an01227f

• ULTRASENSITIVE DETECTION
• SENSITIVE DETECTION
• ENERGY-TRANSFER
• AFLATOXIN B-1
• SENSOR
• CELLS
• ASSAY
• FRET
• CHROMATOGRAPHY
• NANOPARTICLES

Tetrodotoxin (TTX) is a potent, low molecular weight analyte that can lead to fatal poisoning and requires a sensitive, rapid detection method. Here, we have developed a competitive, lateral-flow immunochromato-graphic strip combined with quantum dot nanobeads (QDNBs) and gold nanoflowers (AuNFs). This approach is called turn-on C-LFICS and it meets all testing requirements. Subsequent analysis revealed that this turn-on C-LFICS was rapid (8 min), sensitive (LOD = 0.2 ng mL(-1)), and quantitative (DLR = 1.56-100 ng mL(-1)), and had a positive signal readout (based on fluorescence quenching effects) for TTX detection. Moreover, it had superior signal brightness and a low background interference signal when compared with previous methods. Finally, it can function free of interference from the sample matrix and has a demonstrated recovery range of 85.5% to 119.7% in spiked samples. Taken together, these results show that our turn-on C-LFICS is an effective detection tool for TTX or other small molecules.

• 出版日期2017-12-7
• 单位深圳市计量质量检测研究院; 华南农业大学; 暨南大学