DEGRADATION, RESIDUAL DETERMINATION, AND CHOLINESTERASE ACTIVITY OF TRICLORFON IN Piaractus mesopotamicus HOLMBERG ( PACU) 1887

作者：Mataqueiro Maria Isabel^*; Okada Nakaghi Laura Satiko; Yamada Patricia Kii; Ferraz Guilherme de Camargo; de Queiroz Neto Antonio; DeOliveira Georgino Honorato

来源：Journal of Toxicology and Environmental Health Part A: Current Issues , 2014, 77(1-3): 125-132.

DOI：10.1080/15287394.2013.866928

The aim of this study was to determine the no-observable-adverse-effect concentration (NOAEC) for trichlorfon, an antiparasitic agent used in aquaculture, in Piractus mesopotamicus (pacu) using acetylcholinesterase (AChE) activity as an end point. Fish were exposed 24 h/d for 15 d to different concentrations of trichlorfon in tanks of water for which a curve of dissipation was previously determined. Analysis of trichlorfon in water and fish plasma using gas chromatography with electron capture detection (GC-ECD) enabled measurement of limit of detection (LOD) and limit of quantification (LOQ), respectively, to be 3 and 10 ppb. Thirty-six hours after trichlorfon dilution in water, the concentration was below the LOD, and data showed that plasma concentrations did not exceed the LOQ. Apart from the 6.25 g/L, all concentrations of trichlorfon significantly inhibited plasma and brain AChE activity compared to controls. The AChE activity levels returned to control values in 7 d. These data may be useful to determine the concentration of trichlorfon that destroys parasites without producing adverse effects in fish.

• 出版日期2014-2-1
• 单位Univ Estadual Paulista