A novel library of - arylketones as potential inhibitors of alpha-glucosidase: Their design, synthesis, in vitro and in vivo studies

作者:Luthra Tania; Agarwal Rahul; Estari Mamidala; Adepally Uma*; Sen Subhabrata*
来源:Scientific Reports, 2017, 7(1): 13246.
DOI:10.1038/s41598-017-13798-y

摘要

alpha-glucosidase is an essential enzyme located at the brush border of intestines. It is an important therapeutic target for type II diabetes. Herein we have designed a library of novel alpha-arylketones as inhibitors of alpha-glucosidase (yeast origin) via scaffold hopping and bioisosteric modification of known inhibitors of alpha-glucosidase. The design was validated through molecular docking that revealed strong binding interactions of the newly designed compounds against a-glucosidase. A library comprising of 15 compounds was synthesized in a combinatorial fashion, where the advanced amide intermediates were accessed through "shot gun" synthesis. The final compounds were characterized by H-1, C-13-NMR and with high resolution mass spectroscopy. In vitro screening of the compounds against yeast a-glucosidase revealed substantial inhibition with IC(50)s in the range of 4-10 mu M (the standard drug acarbose inhibits a-glucosidase with an IC50 of 9.95 mu M). Reaction kinetics suggested mixed type inhibition. Finally, in vivo studies of the most active compound 3c against Streptozotocin induced male albino Wistar rats revealed that its administration in the rats for about 4 weeks lead to a highly significant (P < 0.001) decrease in the fasting blood glucose (FBG) compared to the untreated diabetic rats. Moreover, lower dose of 3c had better control over FBG in contrast to high-dose.

  • 出版日期2017-10-16