Different Anesthesia Regimes Modulate the Functional Connectivity Outcome in Mice

作者：Jonckers Elisabeth^*; Delgado y Palacios Rafael; Shah Disha; Guglielmetti Caroline; Verhoye Marleen; Van der Linden Annemie

来源：Magnetic Resonance in Medicine, 2014, 72(4): 1103-1112.

DOI：10.1002/mrm.24990

• alpha-chloralose
• conscious
• functional connectivity
• isoflurane
• mouse
• urethane

PurposeThe use of resting-state functional MRI (rsfMRI) in preclinical research is expanding progressively, with the majority of resting-state imaging performed in anesthetized animals. Since anesthesia may change the physiology and, in particular, the neuronal activity of an animal considerably, it may also affect rsfMRI findings. Therefore, this study compared rsfMRI data from awake mice with rsfMRI results obtained from mice anesthetized with -chloralose (120 mg/kg), urethane (2.5 g/kg), or isoflurane (1%).
MethodsFunctional connectivity (FC) was estimated using both independent component analysis (40 components) and ROI-based analysis to zoom in on the effect of different anesthetics on inter-hemispheric FC.
ResultsThe data revealed an important diminishment of cortical interhemispheric FC in both the -chloralose and urethane groups in comparison with the isoflurane and awake groups.
ConclusionWhen performing FC analysis in anesthetized mice, the impact of anesthetics must be taken into account. The required doses for stable anesthesia during MRI significantly decrease interhemispheric FC. Magn Reson Med 72:1103-1112, 2014.

• 出版日期2014-10