EF-1 alpha and Mitochondrial ATP Synthase beta Chain: Alteration of their Expression in Encystment-Induced Colpoda cucullus

作者：Sogame Yoichiro; Kojima Katsuhiko; Takeshita Toshikazu; Kinoshita Eiji; Matsuoka Tatsuomi^*

来源：Journal of Eukaryotic Microbiology, 2012, 59(4): 401-406.

DOI：10.1111/j.1550-7408.2012.00628.x

• LC-MS
• MS analysis
• cellular morphogenesis
• cyst formation

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the total proteins contained in encystment-induced Colpoda cucullus showed that a 50-kDa protein (p50) disappeared, whereas the expression of a 49-kDa protein (p49) was enhanced in early phase of morphogenetic transformation into the resting cyst (i.e. 2-5 h after the onset of encystment induction). Puromycin or actinomycin D inhibited the alteration in the expression of p50 and p49 by the induction of encystment. These results suggest that the encystment-specific alteration in expression of these proteins is performed by a transcriptional regulation. Liquid chromatography tandem mass spectrometry analysis revealed that p50 is mitochondrial ATP synthase beta chains, and that p49 is elongation factor 1a.

• 出版日期2012-8