科研之友
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摘要
Tissue regeneration and repair have been observed among eukaryotes such as sponges, hydras, planarians and amphibians, but this ability is rare among mammals. In stem cells, the expression of the Oct4 gene inhibits the p21 gene and initiates cell division. In the ear cells of experimental mouse, signs of repair appear 48 hours after sustaining an injury. In cultured cells, employing RT-PCR (Reverse Transcriptase-PCR) and western blotting, Oct4 gene expression methods were observed at both the transcriptional and translational levels. Also, Oct4 gene sequencing was ascertained, which was consistent with sequencing registered in National Center for Biotechnology Information (NCBI). Studying the bands that resulted from RT-PCR reveals Oct4 gene expression in blastoma cells, and the result of western blotting demonstrates the translation of Oct4 protein in these cells. This article reveals the role of Oct4 genes in auricle blastoma cells of mouse in addition to the contribution of stem cells make to tissue regeneration in this animal. This raises hopes that, following sustained sores or the loss of a limb, inducing factors may be released in mice and other mammals that have the ability to transform the cells around a wound to stem cells. This research yields results that may help cure diseases such as leprosy and gangrene.
- 出版日期2011
