The Diagnostic Value of Whole Blood lncRNA ENST00000550337.1 for Pre-Diabetes and Type 2 Diabetes Mellitus

作者：Li, Xuejie; Zhao, Zhenzhou; Gao, Chuanyu; Rao, Lixin; Hao, Peiyuan; Jian, Dongdong; Li, Wentao; Tang, Haiyu; Li, Muwei^*

来源：Experimental and Clinical Endocrinology & Diabetes, 2017, 125(6): 377-383.

DOI：10.1055/s-0043-100018

• diabetes mellitus
• type 2
• pre-diabetes
• RNA
• long noncoding
• microarray analysis
• biological markers

Background This study aims to investigate long noncoding RNA (lncRNA) as biomarker for pre-diabetes and T2DM. @@@ Methods LncRNAs in the peripheral blood of 6 healthy individuals and 6 T2DM patients were collected for microarray analysis. Then 5 candidate biomarkers from the differentially expressed lncRNAs were chosen and verified in a larger independent cohort (control group = 20; pre-diabetes group = 20; and T2DM group = 20). The diagnostic capacity of ENST00000550337.1 was further tested in the third cohort (control group, n = 60; pre-diabetes group, n = 63; and T2DM group, n = 64). @@@ Results A total of 17 lncRNAs were found to be differentially expressed between the 2 groups. 14 lncRNAs of these were upregulated in T2DM patients and 3 were downregulated. 5 upregulated lncRNAs were selected as potential biomarkers and verified in the second cohort, and the expression levels of 3 lncRNAs increased gradually from the control group to the pre-diabetes group to the T2DM group. The diagnostic value of ENST00000550337.1 was then tested in the third cohort, and its high diagnostic value for pre-diabetes and T2DM was confirmed. @@@ Conclusions LncRNA ENST00000550337.1 is a potential diagnostic biomarker for pre-diabetes and T2DM.

• 出版日期2017-6
• 单位浙江大学; 郑州大学