A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro

作者：Chen, Wen-Liang; Qian, Yan; Meng, Wei-Feng; Pang, Ji-Yan; Lin, Yong-Cheng; Guan, Yong-Yuan; Chen, Sheng-Pin; Liu, Jie; Pei, Zhong; Wang, Guan-Lei^*

来源：Biochemical Pharmacology, 2009, 78(8): 941-950.

DOI：10.1016/j.bcp.2009.05.029

• Xyloktals
• Endothelial cells
• Oxidized low-density lipoprotein
• Reactive oxygen species
• Nitric oxide
• Nicotinamide adenine dinucleotide phosphate oxidase

Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Pretreatment with xyloketal B (0.63-40 mu M) significantly improved oxLDL (150 mu g/ml)-induced injury in human umbilical vein endothelial cells (HUVECs) without either toxic or proliferative effects. Xyloketal B concentration-dependently attenuated oxLDL-induced ROS generation, peroxynitrite formation and decrease of Bcl-2 expression. In addition, xyloketal B significantly inhibited NADPH oxidase activity, as well as mRNA expression of gp91phox and p47phox. Furthermore, xyloketal B alone augmented the production of nitric oxide (NO). Collectively, these data indicate that xyloketal B protects against oxLDL-induced endothelial oxidative injury probably through inhibiting NADPH oxidase-derived ROS generation, promoting NO production and restoring Bcl-2 expression, making it a promising compound for further evaluation in the treatment of atherosclerosis.

• 出版日期2009-10-15
• 单位中山大学