A novel fluorescein-coumarin-based fluorescent probe for fluoride ions and its applications in imaging of living cells and zebrafish in vivo

作者：Jiao, Shan; Wang, Xiaochun; Sun, Yihan; Zhang, Lijun; Sun, Weihong; Sun, Ying; Wang, Xinghua; Ma, Pinyi^*; Song, Daqian^*

来源：Sensors and Actuators B: Chemical , 2018, 262: 188-194.

DOI：10.1016/j.snb.2018.01.186

• Fluorescence
• Probe
• Fluoride ions
• Living cells
• Zebrafish

A novel fluorescent probe (Flu-Si) based on fluorescein and coumarin fluorophores for highly selective and sensitive detection of F- was successfully developed. It was shown that the mechanism of recognition involves a desilylation reaction that takes place after addition of F- anions. A linear relationship between the ratio of emission intensities at 532 and 465 nm (I-532/I-465) and F- concentration over the range of 0-20 mu mol L-1 with a LOD of 0.025 mu mol L-1 was found. The sensing mechanism of Flu-Si with F- was confirmed by density functional theory (DFT) and time dependent density functional theory (TDDFT) calculation. Moreover, it was demonstrated that Flu-Si is an efficient fluorescent probe for F- anions in living cells and zebrafish, indicating its sensing potential in clinical diagnosis.

• 出版日期2018-6-1
• 单位吉林大学; 鞍山师范学院