Apoptosis induced by endoplasmic reticulum stress involved in diabetic kidney disease

作者：Liu, Guanghui; Sun, Yingying; Li, Zhenhua; Song, Tao; Wang, Haibin; Zhang, Yun; Ge, Zhiming^*

来源：Biochemical and Biophysical Research Communications, 2008, 370(4): 651-656.

DOI：10.1016/j.bbrc.2008.04.031

• diabetic kidney disease
• apoptosis
• endoplasmic reticulum stress
• albuminuria

Endoplasmic reticulum stress has been suggested to play a crucial role in the pathogenesis of diabetic complications. However, whether it is involved in the renal injury of diabetic nephropathy is still not known. We investigated the involvement of ER-associated apoptosis in kidney disease of streptozocin (STZ)-induced diabetic rats. We used albuminuria examination, hematoxylin & eosin (H&E) staining and TUNEL analysis to identify the existence of diabetic nephropathy and enhanced apoptosis. We performed immunohistochemistry, Western blot, and real-time PCR to analyze indicators of ER molecule chaperone and ER-associated apoptosis. GRP78, the ER chaperone, was up-regulated significantly in diabetic kidney compared to control. Furthermore, three hallmarks of ER-associated apoptosis, C/EBP homologous protein (CHOP), c-JUN NH2-terminal kinase (JNK) and caspase-12, were found to have activated in the diabetic kidney. Taken together, those results suggested that apoptosis induced by ER stress occurred in diabetic kidney, which may contribute to the development of diabetic nephropathy.

• 出版日期2008-6-13
• 单位山东大学