Astragalin Inhibits Allergic Inflammation and Airway Thickening in Ovalbumin-Challenged Mice

作者：Kim, Yun-Ho; Choi, Yean-Jung; Kang, Min-Kyung; Park, Sin-Hye; Antika, Lucia Dwi; Lee, Eun-Jung; Kim, Dong Yeon; Kang, Young-Hee^*

来源：Journal of Agricultural and Food Chemistry, 2017, 65(4): 836-845.

DOI：10.1021/acs.jafc.6b05160

• airway inflammation
• airway smooth muscle
• astragalin
• macrophages
• mast cells
• neutrophils
• oxidative stress

Lung inflammation and oxidative stress are the major contributors to the development of obstructive pulmonary diseases. Macrophages are involved in pulmonary inflammation and alveolar damage in emphysema. Astragalin is an anti-inflammatory flavonoid present in persimmon leaves and green tea seeds. This study elucidated that astragalin inhibited inflammatory cell infiltration induced by 20 mu M H2O2 and blocked airway thickening and alveolar emphysema induced by 20 pig of ovalbumin (OVA) in mice. OVA induced mouse pulmonary MCP-1, and H2O2 enhanced the expression of MCP-1/ICAM-1/ccv integrin in bronchial airway epithelial BEAS-2B cells. Such induction was inhibited by supplying 10-20 mg/kg of astragalin to OVA-challenged mice and 1-20 mu M astragalin to oxidant-stimulated cells. Oral administration of 20 mg/kg of astragalin reduced the induction of F4/80/CD68/CD11b in airways of mice challenged with OVA. Additionally, emphysema tissue damage was observed in OVA-exposed alveoli. Mast cell recruitment in the airway subepithelium was blocked by supplementing astragalin to OVA-challenged mice. Orally treating 20 mg/kg of astragalin reduced alpha-SMA. induction in inflammation-occurring airways and appeared to reverse airway thickening and constriction induced by an OVA episode. These results revealed that astragalin may improve airway thickening and alveolar destruction with blockade of allergic inflammation in airways. Therefore, astragalin may be a therapeutic agent antagonizing asthma and obstructive pulmonary diseases.

• 出版日期2017-2-1