AMINO-ACID-SEQUENCE OF FEL-DI, THE MAJOR ALLERGEN OF THE DOMESTIC CAT - PROTEIN-SEQUENCE ANALYSIS AND CDNA CLONING

作者：MORGENSTERN JP; GRIFFITH IJ; BRAUER AW; ROGERS BL; BOND JF; CHAPMAN MD; KUO MC

来源：Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(21): 9690-9694.

DOI：10.1073/pnas.88.21.9690

• POLYMERASE CHAIN REACTION
• N-GLYCOSYLATION

The complete primary structure of Fel dI (International Union of Immunological Societies nomenclature), the major allergen produced by the domestic cat, Felis domesticus, was determined by protein sequence analysis and cDNA cloning. Protein sequencing of Fel dI from an immunoaffinity-purified extract of house dust revealed that the allergen is composed of two polypeptide chains. Degenerate oligonucleotides derived from the protein sequence were used in polymerase chain reaction amplification of cat salivary gland cDNA to demonstrate that the two chains are encoded by different genes. Chain 1 of Fel dI shares amino acid homology with rabbit uteroglobin, while chain 2 is a glycoprotein with N-linked oligosaccharides.

• 出版日期1991-11