A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

作者：Moskovtsev Sergey I^*; Alladin Naazish; Lo Kirk C; Jarvi Keith; Mullen J Brendan M; Librach Clifford L

来源：Systems Biology in Reproductive Medicine, 2012, 58(3): 142-148.

DOI：10.3109/19396368.2012.667504

• aneuploidy
• sperm DNA damage
• testicular spermatozoa
• TUNEL

Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates in spermatozoa are elevated at the testicular level in infertile patients compared to ejaculates of normal controls. However, essential data regarding aneuploidy rates between ejaculated and testicular spermatozoa in the same individuals is lacking. The purpose of our study was to compare aneuploidy rates at the testicular and post-testicular level from the same patients with persistently high sperm DNA damage. Ejaculates and testicular biopsies were obtained from eight patients with persistently high DNA damage (>30%). Both ejaculated and testicular samples were analyzed for sperm DNA damage and sperm aneuploidy for chromosomes 13, 18, 21, X, and Y. In addition, semen samples from ten normozoospermic men presenting for fertility evaluation served as a control group. A strong correlation between the alteration of spermatogenesis and chromatin deterioration was observed in our study. In the same individuals, testicular samples showed a significantly lower DNA damage compared to ejaculated spermatozoa (14.9% +/- 5.0 vs. 40.6% +/- 14.8, P < 0.05), but significantly higher aneuploidy rates for the five analyzed chromosomes (12.41% +/- 3.7 vs. 5.77% +/- 1.2, P < 0.05). While testicular spermatozoa appear favourable for ICSI in terms of lower DNA damage, this potential advantage could be offset by the higher aneuploidy rates in testicular spermatozoa.

• 出版日期2012-6