Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance

作者：Tickle Jacqueline A; Jenkins Stuart I; Polyak Boris; Pickard Mark R; Chari Divya M^*

来源：Nanomedicine, 2016, 11(4): 345-358.

DOI：10.2217/nnm.15.202

• astrocytes
• cell transplantation
• label dilution
• magnetite
• magnetolabeling
• polymeric particles

Aim: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. Materials & methods: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes +/- static/oscillating magnetic fields to assess labeling efficiency and safety. Results: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. Conclusion: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.

• 出版日期2016