Analysis of larval antigens of Cephalopina titillator in the camel mucus for diagnosis of infestation

作者：Yousef Hesham A^*; Meguid Afaf Abdel; Afify Amira; Hassan Hany M

来源：Biologia, 2016, 71(4): 438-443.

DOI：10.1515/biolog-2016-0051

• Cephalopina titillator
• salivary gland contents
• excretory secretory products
• digestive tract contents
• ELISA
• camel mucus

Serodiagnostic test for the diagnosis of infestation of camels by the camel nasal fly, Cephalopina titillator was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of antibodies in the camel mucus against salivary gland contents (SGc), excretory-secretory products (ESP), digestive tract contents (DTc) antigens from 2nd and 3rd larval instars of C. titillator sampled in summer and winter. ELISA characterization of larval antigens showed that the presence of C. titillator larvae in camel, Camelus dromedarius, resulted in higher antibody titer in mucus samples of infested camels collected in winter than those collected in summer in both L2 and L3. In L2, the most antigenic secretion was SGc > ESP > DTc, but in L3 ESP > SGc > DTc. The antibody titers in camel mucus are affected by the intensity of infestation with C. titillator.

• 出版日期2016-5