Activation of the Class Ib Ribonucleotide Reductase by a Flavodoxin Reductase in Bacillus cereus

作者：Lofstad Marie; Gudim Ingvild; Hammerstad Marta; Rohr Asmund Kjendseth; Hersleth Hans Petter^*

来源：Biochemistry, 2016, 55(36): 4998-5001.

DOI：10.1021/acs.biochem.6b00699

To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with the RNR Mn-2(II) site to generate a tyrosyl radical that is required for catalysis. A native NrdI reductase has not yet been identified. We herein demonstrate through kinetic and spectroscopic studies that an endogenous flavodoxin reductase can function as the NrdI reductase in Bacillus cereus. When the flavodoxin reductase reduces NrdI, tyrosyl radical formation in RNR is promoted under aerobic conditions, significantly increasing the radical yield. Thus, a missing piece of the class Ib RNR NrdI redox pathway has finally been identified.

• 出版日期2016-9-13