A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods

作者：Kitazaki Hirotaro; Mori Takeshi^*; Kang Jeong Hun; Niidome Takuro; Murata Masaharu; Hashizume Makoto; Katayama Yoshiki

来源：Colloids and Surfaces B: Biointerfaces , 2012, 99: 7-11.

DOI：10.1016/j.colsurfb.2011.08.028

• Protein kinase
• Gold nanorod
• Colorimetry
• Drug screening

We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC50 values of several inhibitors of c-Src determined by the present assay are consistent with reported values.

• 出版日期2012-11-1