Absence of Flow-Mediated Vasodilation in the Rabbit Femoral Artery

作者：Clifford P S; Madden J A; Hamann J J; Buckwalter J B; Valic Z^*

来源：Physiological Research, 2010, 59(3): 331-338.

DOI：10.33549/physiolres.931672

• Blood flow
• Conduit vessel
• Endothelium
• Skeletal muscle

The purpose of this study was to determine if there is flow-mediated vasodilation of the femoral artery in response to progressive increases in flow within a physiological range observed in the in vivo experiments. Femoral artery blood flow was determined in conscious rabbits (n=5) using chronically implanted flowprobes. Resting blood flow was 8.3+/-0.6 ml/min and increased to 39.9+/-5.4 ml/min during high intensity exercise. Femoral arteries (n=12, 1705+/-43 mu m outer diameter) harvested from a separate group of rabbits were mounted on cannulas and diameter was continuously monitored by video system. Functional integrity of the endothelium was tested with acetylcholine. The arteries were set at a transmural pressure of 100 mm Hg and preconstricted with phenylephrine to 73+/-3 % of initial diameter. Using a roller pump with pressure held constant, the arteries were perfused intraluminally with warmed, oxygenated Krebs' solution (pH=7.4) over a physiological range of flows up to 35 ml/min. As flow increased from 5 ml/min to 35 ml/min, diameter decreased significantly (p<0.05) from 1285+/-58 mu m to 1100+/-49 mu m. Thus, in vessels with a functional endothelium, increasing intraluminal flow over a physiological range of flows produced constriction, not dilation. Based on these results, it seems unlikely that flow-mediated vasodilation in the rabbit femoral artery contributes to exercise hyperemia.

• 出版日期2010