摘要

A procedure has been proposed for the determination of Fe, Cu and Zn in protein bands with synchrotron radiation X-ray fluorescence (SRXRF) analysis after isoelectric focusing (IEF) separation. Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. Human hemoglobin was separated with IEF, then Fe, Cu and Zn in protein bands were analyzed by SRXRF. The calibration curves can be obtained in a range of 0-8 mu g/g metals and linear relationship between dosage of metals and fluorescent intensity can be observed (r>0.99). The method provides detection limits of 2.43, 1.12 and 0.96 mu g/g for Fe, Cu and Zn, and recoveries of 90.4% and 115.7% for Fe and Zn, respectively. The combined technique of SRXRF and IEF have advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.

  • 出版日期2006-4
  • 单位中国科学院; 忻州师范学院