Analytical protocol for identification of BMAA and DAB in biological samples

作者：Spacil Zdenek; Eriksson Johan; Jonasson Sara; Rasmussen Ulla; Ilag Leopold L^*; Bergman Birgitta

来源：Analyst, 2010, 135(1): 127-132.

DOI：10.1039/b921048b

b-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.

• 出版日期2010