Mechanism of the alpha beta Conformational Change in F-1-ATPase after ATP Hydrolysis: Free-Energy Simulations

作者：Ito Yuko; Ikeguchi Mitsunori^*

来源：Biophysical Journal, 2015, 108(1): 85-97.

DOI：10.1016/j.bpj.2014.11.1853

One of the motive forces for F-1-ATPase rotation is the conformational change of the catalytically active beta subunit due to closing and opening motions caused by ATP binding and hydrolysis, respectively. The closing motion is accomplished in two steps: the hydrogen-bond network around ATP changes and then the entire structure changes via B-helix sliding, as shown in our previous study. Here, we investigated the opening motion induced by ATP hydrolysis using all-atom free-energy simulations, combining the nudged elastic band method and umbrella sampling molecular-dynamics simulations. Because hydrolysis requires residues in the alpha subunit, the simulations were performed with the alpha beta dimer. The results indicate that the large-scale opening motion is also achieved by the B-helix sliding (in the reverse direction). However, the sliding mechanism is different from that of ATP binding because sliding is triggered by separation of the hydrolysis products ADP and Pi. We also addressed several important issues: 1), the timing of the product Pi release; 2), the unresolved half-closed beta structure; and 3), the ADP release mechanism. These issues are fundamental for motor function; thus, the rotational mechanism of the entire F-1-ATPase is also elucidated through this alpha beta study. During the conformational change, conserved residues among the ATPase proteins play important roles, suggesting that the obtained mechanism may be shared with other ATPase proteins. When combined with our previous studies, these results provide a comprehensive view of the beta-subunit conformational change that drives the ATPase.

• 出版日期2015-1-6