Assessing signaling pathways associated with in vitro resistance to cytotoxic agents in AML

作者：Rosen David B; Cordeiro James A; Cohen Aileen; Lacayo Norman; Hogge Donna; Hawtin Rachael E; Cesano Alessandra^*

来源：Leukemia Research, 2012, 36(7): 900-904.

DOI：10.1016/j.leukres.2012.02.022

• Cell signaling
• Acute myeloid leukemia
• Flow cytometry
• Drug resistance
• Single cell network profiling
• Signaling pathways
• Chemoresistance
• Apoptosis
• DNA damage

This study uses single cell network profiling (SCNP) to characterize biological pathways associated with in vitro resistance or sensitivity to chemotherapeutics commonly used in acute myeloid leukemia (AML) (i.e. cytarabine/daunorubicin, gemtuzumab ozogamicin (GO), decitabine, azacitidine, clofarabine). Simultaneous measurements at the single cell level of changes in DNA damage, apoptosis and signaling pathway responses in AML blasts incubated in vitro with the above drugs showed distinct profiles for each sample and mechanistically different profiles between distinct classes of agents. Studies are ongoing to assess the clinical predictive value of these findings.

• 出版日期2012-7