DOC1-Dependent Recruitment of NURD Reveals Antagonism with SWI/SNF during Epithelial-Mesenchymal Transition in Oral Cancer Cells

作者：Mohd Sarip Adone; Teeuwssen Miriam; Bot Alice G; De Herdt Maria J; Willems Stefan M; de Jong Robert J Baatenburg; Looijenga Leendert H J; Zatreanu Diana; Bezstarosti Karel; van Riet Job; Oole Edwin; van Ijcken Wilfred F J; van de Werken Harmen J G; Demmers Jeroen A; Fodde Riccardo; Verrijzer C Peter

来源：Cell Reports, 2017, 20(1): 61-75.

DOI：10.1016/j.celrep.2017.06.020

The Nucleosome Remodeling and Deacetylase (NURD) complex is a key regulator of cell differentiation that has also been implicated in tumorigenesis. Loss of the NURD subunit Deleted in Oral Cancer 1 (DOC1) is associated with human oral squamous cell carcinomas(OSCCs). Here, weshow that restoration of DOC1 expression in OSCC cells leads to a reversal of epithelial-mesenchymal transition (EMT). This is caused by the DOC1-dependent targeting of NURD to repress key transcriptional regulators of EMT. NURD recruitment drives extensive epigenetic reprogramming, including eviction of the SWI/SNF remodeler, formation of inaccessible chromatin, H3K27 deacetylation, and binding of PRC2 and KDM1A, followed by H3K27 methylation and H3K4 demethylation. Strikingly, depletion of SWI/SNF mimics the effects of DOC1 re-expression. Our results suggest that SWI/SNF and NURD function antagonistically to control chromatin state and transcription. We propose that disturbance of this dynamic equilibrium may lead to defects in gene expression that promote oncogenesis.

• 出版日期2017-7-5