A genome-wide association study identifies locus at 10q22 associated with clinical outcomes of adjuvant tamoxifen therapy for breast cancer patients in Japanese

作者：Kiyotani Kazuma; Mushiroda Taisei; Tsunoda Tatsuhiko; Morizono Takashi; Hosono Naoya; Kubo Michiaki; Tanigawara Yusuke; Imamura Chiyo K; Flockhart David A; Aki Fuminori; Hirata Koichi; Takatsuka Yuichi; Okazaki Minoru; Ohsumi Shozo; Yamakawa Takashi; Sasa Mitsunori; Nakamura Yusuke^*; Zembutsu Hitoshi

来源：Human Molecular Genetics, 2012, 21(7): 1665-1672.

DOI：10.1093/hmg/ddr597

Although many association studies of polymorphisms in candidate genes with the clinical outcomes of breast cancer patients receiving adjuvant tamoxifen therapy have been reported, genetic factors determining individual response to tamoxifen are not fully understood. To identify genetic polymorphisms associated with clinical outcomes of patients with tamoxifen treatment, we conducted a genome-wide association study (GWAS). We studied 462 Japanese patients with hormone receptor-positive, invasive breast cancer receiving adjuvant tamoxifen therapy. Of them, 240 patients were analyzed by genome-wide genotyping using the Illumina Human610-Quad BeadChips, and two independent sets of 105 and 117 cases were used for replication studies. In the GWAS, we detected significant associations with recurrence-free survival at 15 single-nucleotide polymorphisms (SNPs) on nine chromosomal loci (1p31, 1q41, 5q33, 7p11, 10q22, 12q13, 13q22, 18q12 and 19p13) that satisfied a genome-wide significant threshold (log-rank P 2.87 10(9)9.41 10(8)). Among them, rs10509373 in C10orf11 gene on 10q22 was significantly associated with recurrence-free survival in the replication study (log-rank P 2.02 10(4)) and a combined analysis indicated a strong association of this SNP with recurrence-free survival in breast cancer patients treated with tamoxifen (log-rank P 1.26 10(10)). Hazard ratio per C allele of rs10509373 was 4.51 [95 confidence interval (CI), 2.727.51; P 6.29 10(9)]. In a combined analysis of rs10509373 genotype with previously identified genetic makers, CYP2D6 and ABCC2, the number of risk alleles of these three genes had cumulative effects on recurrence-free survival among 345 patients receiving tamoxifen monotherapy (log-rank P 2.28 10(12)). In conclusion, we identified a novel locus associated with recurrence-free survival in Japanese breast cancer patients receiving adjuvant tamoxifen therapy.

• 出版日期2012-4-1
• 单位河北医科大学