Application of comprehensive NMR-based analysis strategy in annotation, isolation and structure elucidation of low molecular weight metabolites of Ricinus communis seeds

作者：Vuckovic Ivan; Rapinoja Marja Leena; Vaismaa Matti; Vanninen Paula; Koskela Harri^*

来源：Phytochemical Analysis, 2016, 27(1): 64-72.

DOI：10.1002/pca.2600

• Ricinus communis
• low molecular weight metabolites
• analysis strategy
• NMR
• HPLC
• LC-MS

Introduction - Powder-like extract of Ricinus communis seeds contain a toxic protein, ricin, which has a history of military, criminal and terroristic use. As the detection of ricin in this "terrorist powder" is difficult and time-consuming, related low mass metabolites have been suggested to be useful for screening as biomarkers of ricin.
Objective - To apply a comprehensive NMR-based analysis strategy for annotation, isolation and structure elucidation of low-molecular weight plant metabolites of Ricinus communis seeds.
Methodology - The seed extract was prepared with a well-known acetone extraction approach. The common metabolites were annotated from seed extract dissolved in acidic solution using H-1 NMR spectroscopy with spectrum library comparison and standard addition, whereas unconfirmed metabolites were identified using multi-step off-line HPLC-DAD-NMR approach.
Results - In addition to the common plant metabolites, two previously unreported compounds, 1,3-digalactoinositol and ricinyl-alanine, were identified with support of MS analyses.
Conclusion - The applied comprehensive NMR-based analysis strategy provided identification of the prominent low molecular weight metabolites with high confidence.

• 出版日期2016-2