Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

作者：Brannigan JA; Ashford SR; Doherty AJ; Timson DJ; Wigley DB^*

来源：Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 1999, 1432(2): 413-418.

DOI：10.1016/S0167-4838(99)00122-3

• DNA replication
• DNA ligation
• thermostable protein
• NAD(+)
• Bacillus stearothermophilus

The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD(+)-dependent DNA ligase.

• 出版日期1999-7-13