Association between the mitochondrial DNA 4977 common deletion in the hair shaft and hearing loss in presbycusis

作者：Liu Hong; Han Yuechen; Wang Shuai; Wang Haibo^*

来源：Molecular Medicine Reports, 2015, 11(2): 1127-1131.

DOI：10.3892/mmr.2014.2877

• presbycusis
• human hair shaft
• common deletion
• mitochondria
• 4977-bp deletion

The aim of the present study was to examine the role of the mitochondrial (mt) DNA (common) (deletion) ((CD)) (4977) ((mtDNACD4977)) in the hair shaft in patients with presbycusis. A total of 87 individuals with presbycusis and 95 normal-hearing controls were selected based on strict audiometric criteria. Nested polymerase chain reaction (PCR), sequencing and quantitative (q)PCR were used to examine the expression levels of mtDNA(CD4977) in the hair shaft in presbycusis. Nested PCR of the hair shaft demonstrated that 8/95 cases with normal hearing were found to be positive for mtDNA(CD4977), as compared with 59/87 cases in the presbycusis group. The mtDNA(CD4977) was positive in 22/43 cases with mild-to-moderate hearing loss, 25/31 cases with moderate-to-severe, severe hearing loss, and 12/13 cases with profound deafness. Statistically significant differences in mtDNA(CD4977) expression were identified among all of the groups (P<0.001). The sequencing and qPCR assays demonstrated a trend towards an increase in the mean CD level of mtDNA(CD4977) with a more severe hearing loss at 8 kHz (r=0.778, P<0.001) and all ranges of frequency (r=0.858, P<0.001). In conclusion, the present study demonstrates a correlation between mtDNA(CD4977) in the human hair shaft and the severity of hearing loss in presbycusis.

• 出版日期2015-2
• 单位山东大学