Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots

作者：Ninkovic Slavica^*; Djordjevic Tatjana; Vinterhalter Branka; Uzelac Branka; Cingel Aleksandar; Savic Jelena; Radovic Svetlana

来源：Plant Cell, Tissue and Organ Culture, 2010, 103(1): 81-91.

DOI：10.1007/s11240-010-9757-x

• Sugar beet
• Agrobacterium rhizogenes
• Callus
• TIBA
• Somatic embryogenesis

Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet.

• 出版日期2010-10