Determination of clenbuterol residual by chemiluminescent enzyme immunossay

作者：Xu CL^*; Peng CF; Hao K; Jin ZG; Wang WK

来源：Chinese Journal of Analytical Chemistry, 2005, 33(5): 699-702.

• chemiluminescent enzyme immmunoassy
• clenbuterol
• residual

A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) for the determination of clenbuterol (CLB) residual in urine has been developed. After optimized the four physico-chemical parameters of competitive reactive time, concentration of Tween-20, concentration of phosphate buffer solution (PBS) and its pH, the developed method gave a limit of detection of 0.01 mu g/L and a detection range from 0.04 mu g/L to 25.8 mu g/L, with an ED50 of 0.54 mu g/L. The developed method has been validated on spiked urea samples in terms of precision (intra- and interassay relative standard deviations of less than 7% and 10%, respectively), and of accuracy (mean recovery from 98% to 120%). All these parameters being better than the enzyme linked immunosorbent assay (ELISA) method which is widely used to detect determination of clenbuterol now and it indicats that the CLEIA method can be used to analyze urea sample instead of ELSIA.

• 出版日期2005-5
• 单位江南大学