Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

作者：Cordeiro Rossana de Aguiar^*; Nobre Patoilo Kharla Rabelo; Praciano Silviane Bandeira; Astete Medrano Delia Jessica; de Farias Marques Francisca Jakelyne; Soares Martins Liline Maria; Eulalio Kelsen Dantas; de Deus Filho Antonio; Salmito Cavalvanti Maria do Amparo; Bezerra Fechine Maria Auxiliadora; Nogueira Brilhante Raimunda Samia; de Camargo Zoilo Pires; Gadelha Rocha Marcos Fabio; Costa Sidrim Jose Julio

来源：Mycopathologia, 2013, 175(1-2): 25-32.

DOI：10.1007/s11046-012-9604-1

• Coccidioidomycosis
• Diagnosis
• Antigen
• Immunodiffusion
• Enzyme immunoassay

Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America.

• 出版日期2013-2