A mammalian insulysin homolog is regulated by enzyme IIA(Glc) of the glucose transport system in Vibrio vulnificus

作者：Kim You Jin; Ryu Yangkyun; Koo Byoung Mo; Lee Na Yeon; Chun Se Jin; Park Soon Jung; Lee Kyu Ho; Seok Yeong Jae^*

来源：FEBS Letters, 2010, 584(22): 4537-4544.

DOI：10.1016/j.febslet.2010.10.035

• Enzyme IIA(Glc)
• Insulysin
• Phosphoenolpyruvate:sugar phosphotransferase system
• Protein-protein interaction
• Vibrio vulnificus

Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus PTS. Here we show that enzyme IIA(Glc) of the PTS regulates the peptidase activity of a mammalian insulysin homolog in V. vulnificus. While interaction of IIA(Glc) with the insulysin homolog is independent of the phosphorylation state of IIA(Glc), only unphosphorylated IIA(Glc) activates the insulysin homolog. Taken together, our results suggest that the V. vulnificus insulysin-IIA(Glc) complex plays a role in survival in the host by sensing glucose.
Structured summary:
MINT-8045996:
IIA glu (uniprotkb:Q7MBY2) binds (MI:0407) to vIDE (uniprotkb:Q7MIS6) by pull down (MI: 0096)
MINT-8045817, MINT-8045967:
IIA glu (uniprotk

• 出版日期2010-11-19