alpha-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin

作者：Prudner Bethany C; Roy Pritam Sinha; Damron Derek S; Russell Mary A^*

来源：FEBS LETTERS, 2014, 588(24): 4625-4630.

DOI：10.1016/j.febslet.2014.11.001

• Synemin
• A-kinase anchoring protein
• Protein kinase A
• Intermediate filament
• Signal transduction
• Cytoskeleton

alpha-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein-protein interaction that regulates the subcellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere.

• 出版日期2014-12-20