A rat model of oral submucous fibrosis induced by bleomycin

作者：Zhang, Shan-Shan; Gong, Zhao-Jian; Xiong, Wei; Wang, Xiao; Min, Qian; Luo, Chong-Dai; Ling, Tian-You^*

来源：Oral Surgery Oral Medicine Oral Pathology Oral Radiology, 2016, 122(2): 216-223.

DOI：10.1016/j.oooo.2015.07.042

• EXTRACELLULAR-MATRIX
• WISTAR RATS
• BETEL-QUID
• INDUCTION
• MYOFIBROBLAST
• CAPSAICIN
• PALATE

Objective. The aim of this study was to induce a rat model of oral submucous fibrosis (OSF) using bleomycin. Study Design. A randomized trial was performed. Bleomycin dilution or phosphate-buffered saline was injected daily into the buccal mucosa of the test and control rats, respectively, for 2, 4, 6, or 8 weeks. Changes in histopathologic features, myofibroblasts, ultrastructure, and the levels of collagen type I and III, transforming growth factorebeta 1, and interferon-g were measured. @@@ Results. Compared with controls, the buccal mucosa of the test rats appeared pale and stiff. There was collagen deposition in the laminae propria. Type I and type III collagen increased steadily, transforming growth factorebeta 1 increased and then decreased, and interferon-g was gradually reduced. The ultrastructure was also similar to that of OSF. @@@ Conclusions. Using bleomycin, we induced a rat model of OSF that is very similar to human OSF in clinical manifestations, pathology, and ultrastructural changes.

• 出版日期2016-8
• 单位中南大学