Dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (INA) or AMT, a psoralen compound (4 '-aminomethyl-trioxsalen) is highly immunogenic in mice

作者：Raviprakash Kanakatte^*; Sun Peifang; Raviv Yossef; Luke Thomas; Martin Nicholas; Kochel Tadeusz

来源：Human Vaccines & Immunotherapeutics, 2013, 9(11): 2336-2341.

DOI：10.4161/hv.25602

• dengue virus
• virus inactivation
• vaccine
• immunogenicity
• mouse

Two novel methods of dengue virus inactivation using iodonaphthyl azide (INA) and aminomethyl trioxsalen (AMT) were compared with traditional virus inactivation by formaldehyde. The AMT inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and INA led to 30-50% decrease in binding. All three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. However, only mice vaccinated with AMT inactivated virus mounted T cell responses similar to live, uninactivated virus.

• 出版日期2013-11