Aluminium-induced effects on growth, morphogenesis and oxidative stress reactions in in vitro cultures of quince

作者：Schuch Marcia Wulff; Cellini Antonio; Masia Andrea; Marino Grazia^*

来源：Scientia Horticulturae, 2010, 125(2): 151-158.

DOI：10.1016/j.scienta.2010.03.007

• Catalase (CAT)
• Caulogenesis
• Cydonia oblonga L.
• Malondialdehyde (MDA)
• Shoot culture
• Superoxide dismutase (SOD)

The effects of Al(3+) [supplied as Al(2)(SO(4))(3)center dot 18H(2)O] addition to culture media (pH 4.0) on growth, morphogenesis (in leaf explants), and oxidative stress reactions in in vitro cultures of 'BA 29' quince were investigated. Aluminium (Al 0.5 mM) strongly inhibited shoot growth in the proliferation and rooting phases (Al 2.2 mM), reduced shoot proliferation (Al 1.1 mM), and induced tissue browning. Superoxide dismutase (SOD) activity was increased in shoot cultures supplemented with 2 mM Al. Malondialdehyde (MDA) content of shoots was strongly increased by Al during proliferation (starting from Al 1.7 mM) and rooting (already at Al 1.1 mM), thus serving as a good 'marker' for Al toxicity. Even a low concentration of Al (0.5 mM) in the shoot induction medium was found to inhibit shoot regeneration. When standard (Al 0) shoot induction medium was used, leaf explant growth was only reduced by 2.2 mM Al in the subsequent growth phases. Following a preliminary selection for their growth on Al-enriched media, 82 potentially Al-tolerant quince somaclones were selected for further trials.

• 出版日期2010-6-3