Determination of pinostrobin in rat plasma by LC-MS/MS: Application to pharmacokinetics

作者：Hua Xin; Fu Yu Jie^*; Zu Yuan Gang; Zhang Lin; Wang Wei; Luo Meng

来源：Journal of Pharmaceutical and Biomedical Analysis, 2011, 56(4): 841-845.

DOI：10.1016/j.jpba.2011.07.038

• Cajanus cajan
• Pinostrobin (PI)
• LC-MS/MS
• Pharmacokinetics

A rapid and sensitive method for the determination of pinostrobin in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the first time. Isoliquiritigenin was used as an internal standard in rat plasma. Chromatographic separation was performed on an HiQ Sil C(18) column with isocratic elution at a flow rate of 1 mL/min. The mobile phase consisted of water and methanol (9:91, v/v) containing 0.1% formic acid. The quantification limit was 10 ng/mL within a linear range of 10-1000 ng/mL (R = 0.9984). The intra- and inter-day assay precision ranged from 3.8-5.3% to 3.2-5.2%, respectively, and the intra- and inter-day assay accuracy was between 93.2-95.1% and 95.5-104.3%, respectively. Our results indicated that the LC-MS/MS method is effective for pharmacokinetic study of pinostrobin in rat plasma.

• 出版日期2011-12-5
• 单位东北林业大学