Molecular characterization of Neisseria meningitidis isolates recovered from 11-19-year-old meningococcal carriers in Salvador, Brazil

作者：Silva Simoes Moura Ana Rafaela; Kretz Cecilia Batmalle; Ferreira Italo Eustaquio; Pithon Borges Nunes Amelia Maria; de Moraes Jose Cassio; Reis Mitermayer Galvao; Alexander McBride Alan John; Wang Xin; Campos Leila Carvalho

来源：PLos One, 2017, 12(9): e0185038.

DOI：10.1371/journal.pone.0185038

Characterization of meningococci isolated from the pharynx is essential towards understanding the dynamics of meningococcal carriage and disease. Meningococcal isolates, collected from adolescents resident in Salvador, Brazil during 2014, were characterized by multilocus sequence typing, genotyping or whole-genome sequencing. Most were nongroupable (61.0%), followed by genogroups B (11.9%) and Y (8.5%). We identified 34 different sequence types (STs), eight were new STs, distributed among 14 clonal complexes (cc), cc1136 represented 20.3% of the nongroupable isolates. The porA and fetA genotypes included P1.18,25 - 37 (11.9%), P1.18-1,3 (10.2%); F5-5 (23.7%), F4-66 (16.9%) and F1-7 (13.6%). The porB class 3 protein and the fHbp subfamily A (variants 2 and 3) genotypes were found in 93.0 and 71.0% of the isolates, respectively. NHBA was present in all isolates, and while most lacked NadA (94.9%), we detected the hyperinvasive lineages B: P1.19,15: F5-1: ST-639 (cc32); C: P1.22,14 - 6: F3-9: ST-3780 (cc103) and W: P1.5,2: F1-1: ST-11 (cc11). This is the first report on the genetic diversity and vaccine antigen prevalence among N. meningitidis carriage isolates in the Northeast of Brazil. This study highlights the need for ongoing characterization of meningococcal isolates following the introduction of vaccines and for determining public health intervention strategies.

• 出版日期2017-9-20